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| 09.04.2021 | 18:15 | FK Pardubice | - | Bohemians Praha 1905 | 0:2 (0:1) | |
| 10.04.2021 | 14:00 | FK Jablonec | - | FC Fastav Zlín | 3:1 (0:0) | |
| 14:00 | 1. FK Příbram | - | Sigma Olomouc | 0:2 (0:0) | ||
| 14:00 | MFK Karviná | - | Viktoria Plzeň | 1:1 (1:0) | ||
| 16:00 | Baník Ostrava | - | SFC Opava | 3:0 (2:0) | ||
| 18:30 | 1. FC Slovácko | - | Slovan Liberec | 1:0 (0:0) | ||
| 11.04.2021 | 14:00 | Dynamo České Budějovice | - | Zbrojovka Brno | 0:2 (0:0) | |
| 16:00 | FK Teplice | - | FK Mladá Boleslav | 1:3 (1:1) | ||
| 18:30 | Slavia Praag | - | Sparta Praag | 2:0 (1:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praag | 26 | 21 | 5 | 0 | 72:15 | 57 | 68 |
| 2 |
|
Sparta Praag | 26 | 17 | 3 | 6 | 57:30 | 27 | 54 |
| 3 |
|
FK Jablonec | 26 | 16 | 4 | 6 | 44:28 | 16 | 52 |
| 4 |
|
1. FC Slovácko | 26 | 15 | 5 | 6 | 47:26 | 21 | 50 |
| 5 |
|
Viktoria Plzeň | 26 | 13 | 5 | 8 | 45:31 | 14 | 44 |
| 6 |
|
Slovan Liberec | 26 | 12 | 7 | 7 | 37:23 | 14 | 43 |
| 7 |
|
FK Pardubice | 26 | 11 | 5 | 10 | 27:34 | -7 | 38 |
| 8 |
|
Baník Ostrava | 26 | 10 | 7 | 9 | 36:25 | 11 | 37 |
| 9 |
|
Sigma Olomouc | 26 | 9 | 10 | 7 | 32:26 | 6 | 37 |
| 10 |
|
Bohemians Praha 1905 | 26 | 8 | 9 | 9 | 28:28 | 0 | 33 |
| 11 |
|
Dynamo České Budějovice | 26 | 7 | 10 | 9 | 27:35 | -8 | 31 |
| 12 |
|
MFK Karviná | 26 | 7 | 9 | 10 | 28:39 | -11 | 30 |
| 13 |
|
FC Fastav Zlín | 26 | 8 | 5 | 13 | 26:36 | -10 | 29 |
| 14 |
|
FK Mladá Boleslav | 26 | 5 | 9 | 12 | 32:43 | -11 | 24 |
| 15 |
|
FK Teplice | 26 | 6 | 5 | 15 | 28:56 | -28 | 23 |
| 16 |
|
Zbrojovka Brno | 26 | 4 | 8 | 14 | 21:39 | -18 | 20 |
| 17 |
|
SFC Opava | 26 | 3 | 6 | 17 | 16:52 | -36 | 15 |
| 18 |
|
1. FK Příbram | 26 | 2 | 8 | 16 | 17:54 | -37 | 14 |
| Champions League Kwali. | |
| Europa League Kwali. | |
| Degradatie |
